G2025

Sigma

 

Glycerol

cell culture tested, insect cell culture tested, ~99% (GC)

Synonym:1,2,3-Propanetriol, Glycerin
CAS Number:56-81-5
Linear Formula:HOCH2CH(OH)CH2OH
Molecular Weight:92.09
Beilstein Registry Number:635685
EC Number:200-289-5
MDL number:MFCD00004722
PubChem Substance ID:24895082

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Description

ApplicationGlycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel’s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.

Properties

vapor density3.1 (vs air)
vapor pressure<1 mmHg ( 20 °C)
assay~99% (GC)
autoignition temp.698 °F
refractive indexn20/D 1.474(lit.)
EQP levelPremium
bp182 °C/20 mmHg(lit.)
mp20 °C(lit.)
density1.25 g/mL(lit.)
suitabilitycell culture tested
 insect cell culture tested

Safety

Personal Protective EquipmentEyeshields, Gloves, half-mask respirator (EU), half-mask respirator (US), multi-purpose combination respirator cartridge (US)
WGK Germany1
RTECSMA8050000
LEL0.90%(V)
Flash Point(F)320 °F
Flash Point(C)160 °C

References

reference, Andrews, A.T. Electrophoresis: Theory, Techniques, and Biochemical and Clinical Applications 2nd ed., New York , (1988), 252
 , Link, A.J. ed., 2-D Proteome Analysis Protocols. Methods Mol. Biol. Totowa, NJ , (1999) 112
MerckMerck 13,4497
BeilsteinBeil. 1,IV,2751
 Aldrich MSDS 1, 993:A / Corp MSDS 1 (1), 1782:D / FT-IR 2 (1), 265:B / FT-IR 1 (1), 183:A / FT-NMR 1 (1), 283:C / IR-Spectra (2), 103:G / IR-Spectra (3), 112:A / NMR-Reference 2 (1), 171:B / RegBook 1 (1), 185:A / Sax 6, 1479 / Sigma FT-IR 1 (1), 1340:B / Structure Index 1, 24:C:7 / Vapor Phase 3, 262:A